santa cruz biotecnology  (Santa Cruz Biotechnology)

Journal: Particle and Fibre Toxicology

Article Title: Multi-walled carbon nanotubes directly induce epithelial-mesenchymal transition in human bronchial epithelial cells via the TGF-β-mediated Akt/GSK-3β/SNAIL-1 signalling pathway

doi: 10.1186/s12989-016-0138-4

Figure Lengend Snippet: Akt/GSK-3β/SNAIL-1 signalling pathway in BEAS-2B cells exposed to MWCNTg. Cells were incubated in either the absence (0 μg/ml, control) or presence of 44 μg/ml of MWCNTg from 30 min to 8 h ( a ), together with the specific GSK-3β inhibitor SB216763 ( b ), GSK-3β siRNA ( c ), the specific Akt 1/2 Kinase Inhibitor ( d ) or Akt 1/2 siRNA ( e ). Each figure is representative of three experiments giving similar results

Article Snippet: After 24 h, cells were washed once with fresh medium and transfected for 72 h with Akt 1/2 siRNA 50 nM (sc-43609; Santa Cruz Biotechnology Inc.), or GSK-3β siRNA 10 nM (sc-35527; Santa Cruz Biotechnology Inc.), or TGF-β siRNA 10 nM (sc-44146; Santa Cruz Biotechnology Inc.), according to manufacturer’s instructions.

Techniques: Incubation, Control

Journal: Particle and Fibre Toxicology

Article Title: Multi-walled carbon nanotubes directly induce epithelial-mesenchymal transition in human bronchial epithelial cells via the TGF-β-mediated Akt/GSK-3β/SNAIL-1 signalling pathway

doi: 10.1186/s12989-016-0138-4

Figure Lengend Snippet: Akt and GSK-3β inhibition effect in BEAS-2B cells exposed to MWCNTg. The cells were incubated in either the absence (0 μg/ml, control) or presence of 44 μg/ml of MWCNTg for 96 h, without and with 5 μM SB216763 or 10 nM GSK-3β siRNA ( a ), without and with 5 μM of the specific Akt 1/2 Kinase Inhibitor or 50 nM Akt 1/2 siRNA ( b ). Each figure is representative of three experiments giving similar results. Results were expressed in units of relative protein expression compared to control cells (indicated in red in the figure)

Article Snippet: After 24 h, cells were washed once with fresh medium and transfected for 72 h with Akt 1/2 siRNA 50 nM (sc-43609; Santa Cruz Biotechnology Inc.), or GSK-3β siRNA 10 nM (sc-35527; Santa Cruz Biotechnology Inc.), or TGF-β siRNA 10 nM (sc-44146; Santa Cruz Biotechnology Inc.), according to manufacturer’s instructions.

Techniques: Inhibition, Incubation, Control, Expressing

Journal: Particle and Fibre Toxicology

Article Title: Multi-walled carbon nanotubes directly induce epithelial-mesenchymal transition in human bronchial epithelial cells via the TGF-β-mediated Akt/GSK-3β/SNAIL-1 signalling pathway

doi: 10.1186/s12989-016-0138-4

Figure Lengend Snippet: TGF-β production ( a - b ) and TGF-β inhibition effect in BEAS-2B cells exposed to MWCNT ( c ). Cells were incubated for 96 h in either the absence (0 μg/ml, control) or presence of pMWCNT (white square) or MWCNTg (black square) at the concentration of 5.5, 11, 22 and 44 μg/ml ( a ) or incubated up to 24 h in either the absence (0 μg/ml, control) or presence of 44 μg/ml MWCNTg ( b ). The TGF-β production was evaluated by ELISA in the supernatants of cells exposed to MWCNT. Results were expressed as relative fold change compared to control cells ( n = 3). No significant variation was observed in control cells at the different time points evaluated. a Versus control * p < 0.05, ** p < 0.0001; Versus control ° p < 0.05, °° p < 0.0001. b Versus control * p < 0.05, ** p < 0.0001. c The cells were incubated in either the absence (0 μg/ml, control) or presence of 44 μg/ml of MWCNTg for 96 h, without and with 5 μg/ml of neutralizing anti-TGF-β antibody or 10 nM TGF-β siRNA. The efficacy of TGF-β siRNA was evaluated by semi-quantitative PCR. Each figure is representative of three experiments giving similar results. Results were expressed in units of relative protein expression compared to control cells (indicated in red in the figure)

Article Snippet: After 24 h, cells were washed once with fresh medium and transfected for 72 h with Akt 1/2 siRNA 50 nM (sc-43609; Santa Cruz Biotechnology Inc.), or GSK-3β siRNA 10 nM (sc-35527; Santa Cruz Biotechnology Inc.), or TGF-β siRNA 10 nM (sc-44146; Santa Cruz Biotechnology Inc.), according to manufacturer’s instructions.

Techniques: Inhibition, Incubation, Control, Concentration Assay, Enzyme-linked Immunosorbent Assay, Real-time Polymerase Chain Reaction, Expressing